Fig. 5

The effects of PD-1 blockade on tumor growth and T cells in Tyr^+/+ B16-F10 tumors. A The tumor weight of Tyr^+/+ B16-F10 cell grafted Pdcd1^−/− and B6 mice. B Quantification of tumor-infiltrating T cells by flow cytometry in Tyr^+/+ B16-F10 tumors from Pdcd1^−/− and B6 mice. C Representative flow cytometry analysis of CTLA-4⁺ cells in Tyr^+/+ B16-F10 tumors from Pdcd1^−/− and B6 mice. Cells were identified based on CD45⁺, TCR-β⁺, CD4⁺, and CD8⁺ markers. D Quantification of the frequency of CTLA-4⁺ cells within CD4⁺ and CD8⁺ T cells in Tyr^+/+ B16-F10 tumors from Pdcd1^−/− and B6 mice. E–F Flow cytometric analysis of Foxp3 expression in Tyr^+/+ B16-F10 tumors from Pdcd1^−/− and B6 mice. Proportion of Foxp3 in CD4⁺ T cells of Tyr^+/+ B16-F10 tumors from Pdcd1^−/− and B6 mice (F). Data are presented as mean ± SEM (B6 mice, n = 6; Pdcd1^−/− mice, n = 8). Statistical significances were calculated using Student’s t-test (ns, not significant)