Fig. 4

Altered cold-tumor immunophenotype revealed by single-cell RNA sequencing in tyrosinase-deficient B16 melanoma. A q-PCR analyses of Tbx21, Nr4a1, TNF-α and IFN-γ mRNAs in WT and Tyr^−/− B16-F10 tumors (WT tumors, n = 5; Tyr^−/− tumors, n = 5). Each point represents the mean value obtained from two replicates for an individual mouse. Statistical significances were calculated using Student’s t-test (* p < 0.05; ** p < 0.01). B The expression levels and distribution of CD3 in WT and Tyr^−/− tumors demonstrated by t-SNE. C t-SNE visualization of the expression of IL-2, Tbx21, Nr4a1, TNF-α, and IFN-γ in CD4 cells (cluster 5). D t-SNE visualization of the expression of IL-2, Tbx21, Nr4a1, TNF-α, and IFN-γ in CD8 cells (cluster 10)