Fig. 1
From: Tyrosinase in melanoma inhibits anti-tumor activity of PD-1 deficient T cells
Generation of Tyr knockout B16 melanoma cells by CRISPR/Cas9. A Elevated Tyr expression in SKCM tumor tissues compared to normal tissues. The data were obtained from the GEPIA2 database (http://gepia2.cancer-pku.cn). T: SKCMtumor tissues, N: Normal tissues. (*p < 0.05). B Analysis of the correlation between Tyr expression and clinical prognosis in SKCM patients, indicated by overall survival rates from the GEPIA2 database. C Illustration of sgRNA sequences targeting Tyr gene in B16-F10 cells. A visual depiction of the three guide RNA sequences designed to target exon 1 of the Tyr gene in B16-F10 cells is presented in this schematic. The protospacer adjacent motifs (PAMs) are highlighted in red. D Identification of Tyr knockout clones in B16-F10 cells through PCR screening. The three deletion clones are identified as C1, C2, and C3. M referred to the DNA size marker, WT referred to the wild-type B16-F10 control, and H2O was used as a negative control for PCR amplification. E Visual comparison of pigmentation in wild-type (WT) and Tyr knockout (KO) B16-F10 cells