Fig. 1

Selection of gene signatures of oxytosis/ferroptosis and validation in cell culture models of oxytosis/ferroptosis. A Overlap of the FerrDb, TrioSig, and Ferroptosis signatures. B Enrichment of the FerrDb, TrioSig, Ferroptosis, Apoptosis, Necroptosis, and Autophagy signatures in three transcriptomic measures of human and mouse brain cell type-relative expression, including absolute expression (the cell type-specific genes are selected based on their relative expression in a particular cell type irrespective of that in other cell types), enrichment (the genes are selected by measuring the expression of each gene relative to the expression of that gene in all other cell types), and specificity (the genes are defined by the expression of each gene relative to the highest expression of that gene in all other cell types). C Enrichment of the FerrDb, TrioSig, and Ferroptosis signatures in transcriptomic data (DE genes between control and toxicity) from cell culture models where oxytosis/ferroptosis was activated: the original human microglia, astrocytes, and neurons used to generate the TrioSig signature, HT22 mouse hippocampal nerve cells exposed to glutamate, and MC65 human neuroblastoma cells induced to aggregate Aβ intracellularly. Enrichment of Apoptosis, Necroptosis, and Autophagy was also assessed. All DE genes together as well as separated by direction of expression (Down and Up) were analyzed