Fig. 5
Cytotoxicity effect and apoptosis of A549 cells pre-treated with P. aeruginosa PA01 over 14 h. A549 cells were infected with P. aeruginosa at a MOI of 40. Infection progress was monitored by measuring calcein staining intensity using the Operetta screening system; the signals were normalized to those obtained from equivalent reactions without bacterial component (A). All experiments were performed in triplicate. The effect reflected reduced adhesion of P. aeruginosa PA01 in the human A549 lung cell model (B). Adhesion of pre-treated P. aeruginosa PA01 (107 CFU/ml) to A549 cells following 1 h of incubation. Excess bacteria were removed, and the released cells were plated to determine CFU counts. Differences were analyzed for their significance by using one-way ANOVA with Tukey’s test. ***, P < 0.0001