Fig. 4

Inhibition of virulence factor production in P. aeruginosa PA01 grown in the presence of 50 µM NN or DIV (A). Tetracycline treatment was used as positive control. Results are percent of inhibition distinctive to each factor and normalized to bacterial growth at OD600 nm. Error bars represent SD of three independent repetitions. Asterisks indicate significant differences when compared to tetracycline. C. elegans killing assay by P. aeruginosa PA01 assessed by SYTOX Orange stain over 24 h (B). Significance was determined by using one-way ANOVA with Tukey’s test. *** P < 0.0001, ** P < 0.001, and * P < 0.01