Fig. 3
Destruction of P. aeruginosa PA01 mature biofilm and sensitivity to antibiotics. CLSM of biofilm formed in a flow cell after 120 h of incubation at 37 °C (A), after 72 h of incubation alone and then 48 h of incubation in the presence of 50 μM NN (B), 20 μg/ml Tobramycin (C), or 50 μM NN with 20 μg/ml tobramycin (D). Biofilms were stained with the Live/Dead bacterial viability kit. Quantification of bio-volume (E): live, dead, and total bio-volumes (μm3/μm2) were calculated based on image analysis and data from IMARIS software. For each treatment, the images were acquired from three different areas. Results that were significantly different from the control based on a one-way ANOVA with Tukey’s test are labeled with *** P < 0.0001 and * P < 0.01