Fig. 2
Prevention of biofilm formation. Confocal laser scanning micrographs of P. aeruginosa PA01 (A, C, E) and A. baumannii (B, D, F) formed in glass-bottomed 96-well plates after 18 h of static incubation at 37 °C. Cultures were grown in the presence of 50 μM of either DIV or NN or an equivalent amount of DMSO for control. Biofilms were stained using the Live/Dead bacterial viability kit. Only the live cells are represented since the dead fraction was not significant between the groups. Live, dead, and total bio-volumes (μm3/μm2) were calculated based on image analysis and data from the IMARIS software for (G) P. aeruginosa PA01 and (H) A. baumannii. Bars indicate standard deviations for triplicate sets of experiments. Differences were analyzed for their significance by using one-way ANOVA with Tukey’s test. ** P < 0.001 and * P < 0.01