Fig. 9

Dietary inulin supplementation reduced forebrain microgliosis. Illustration of microglia distribution by IBA1 antibody in rostral (A–C) and caudal (G–I) parts of the brain of WT (A, G), Ctrl_AD (B, H), and Inulin_AD (C, I) mice. MC, medial cortex; LC, lateral cortex; VC, ventral cortex; HC, hippocampus; TH, thalamus; MB, midbrain. Scale bar, 500 µm. D–F, J–L Quantification of the percentage of IBA1 area in different brain regions. Comparisons in different regions of cortex were performed by two-way ANOVA with Sidak’s multiple comparisons test (D, J). Unpaired t-test was used to assess the other brain regions (E–F, K–L). P values that were less than 0.2 were indicated. n = 3 in WT group, n = 10 in Ctrl_AD and Inulin_AD groups, represented by a dot in the graphs. Data were presented in mean ± SD. M Western blot and quantification of IBA1 level in the forebrain region of WT, Ctrl_AD, and Inulin_AD mice. Unpaired t-test was used to determine statistical significance between Ctrl_AD and Inulin_AD groups. n = 3 in WT group, n = 5 in Ctrl_AD and Inulin_AD groups, represented by a dot in the graphs. Data were presented in mean ± SD. Supporting data values for D–F, J–K, L–M were included in Additional file 6: Supporting data values. Original images of A–C, G–I, M were included in Additional file 7: original images