Fig. 7

Dietary inulin supplementation reduced forebrain Aβ plaque burden. Illustration of Aβ plaque deposition by 6E10 antibody against human Aβ peptide in rostral (A–C) and caudal (G–I) parts of the brain of WT (A, G), Ctrl_AD (B, H), and Inulin_AD (C, I) mice. MC, medial cortex; LC, lateral cortex; VC, ventral cortex; HC, hippocampus; TH, thalamus; MB, midbrain. Scale bar, 500 µm. D–F, J–L Quantification of the percentage of Aβ area in different brain regions. Comparisons in different regions of cortex were performed by two-way ANOVA with Sidak’s multiple comparisons test (D, J). Unpaired t-test was used to assess the other brain regions (E–F, K–L). P values that were less than 0.2 were indicated. n = 10 in all groups, represented by a dot in the graphs. Data were presented in mean ± SD. M Western blot and quantification of Aβ level in the forebrain region of Ctrl_AD and Inulin_AD mice. Integral density of Aβ 16 and 8 kDa bands was added for quantification. Unpaired t-test was used to determine statistical significance. *, P < 0.05. n = 5 in both groups, represented by a dot in the graphs. Data were presented in mean ± SD. N–O Training latency (time to find the escape tunnel) and percentage of time spent in the target quadrant of the Barnes maze test 3 h after the last training. Data were presented in mean ± SEM. n = 19, 13, and 11 mice for the Ctrl_WT, Ctrl_AD, and Inulin_AD groups, respectively, represented by a dot in the histograms. Two-way ANOVA with Sidak’s multiple comparisons test and one-way ANOVA with Sidak’s multiple comparisons test were used in M and N, respectively. Supporting data values for D–F, J–K, L–O were included in Additional file 6: Supporting data values. Original images of A–C, G–I, M were included in Additional file 7: original images