Fig. 10

Prolonged dietary inulin supplementation reduced Aβ plaque burden in hippocampus. A Illustration of Aβ plaque deposition in hippocampus of Ctrl_AD and Inulin_AD animals at 14-week-old. Scale bar, 200 µm. Illustration and quantification of Aβ plaque deposition in 14- (B) and 36-week-old hippocampus (C), and microgliosis in 36-week-old hippocampus (D). 6E10 and IBA1 staining was performed on the same section in C–D. Unpaired t-test was used to determine statistical significance. *, P < 0.05. n = 3 in Ctrl_AD group, n = 5 in Inulin_AD group, represented by a dot in the graphs. Data were presented in mean ± SD. Scale bar, 200 µm. Western blots and quantification of Aβ level in hippocampus (E) and cortex (F) of Ctrl_AD and Inulin_AD mice. Intensity of Aβ 16 and 8 kDa bands was added for quantification. Unpaired t-test was used to determine statistical significance. ***, P < 0.001. n = 5 in Ctrl_AD group, n = 6 in Inulin_AD group, represented by a dot in the graphs. Data were presented in mean ± SD. Supporting data values for B–F were included in Additional file 6: Supporting data values. Original images of A–F were included in Additional file 7: original images