Fig. 2

Guanine quadruplexes are enriched upstream of POLRMT pause sites. A, B Predicted G4 forming sequences were identified by two algorithms, G4Hunter (A) or QGRS mapper (B) at the 465 POLRMT pause sites or 481 random locations without paused POLRMT. Scores on the Y-axis reflect the strength of G4 prediction. C G4 abundance determined experimentally by BG4 immunoprecipitation followed by sequencing is plotted for the 465 POLRMT pause sites or 481 random sequences without paused POLRMT. In A-C average values are shown and error bands represent S.E.M. D Tracks showing locations of predicted G4 forming sequences by G4Hunter and QGRS mapper (black tracks), G4 enrichment measured by BG4 or mitoBG4 immunoprecipitation followed by sequencing (green tracks), and POLRMT location measured by PRO-seq (blue tracks; RPGC = reads per genomic region mapped, RPM = reads per million, FB = fibroblast, interval shown ChrM:5650–6450)