Fig. 8

Creation of a new binding site for the POU/homeodomain transcriptional regulator, Nubbin, resulting from the mutations in the CYP321B1 promoter region from the resistant strain, enhances the transcriptional level of this gene. A Alignment of CYP321B1 promoter sequences from the resistant and susceptible strains. Two mutation regions (M1 and M2) are marked under the corresponding nucleotides. B The luciferase activity of recovery mutation constructs in the region from −258 to −210. Data are the mean ± SD of three independent assays. Different letters on the right of error bars indicate significant differences based on ANOVA with Tukey’s HSD multiple comparison test (p < 0.05). C Transcription factor CncC/Maf and cis-acting mutation coordinately enhance the promoter activity of CYP321B1 gene. The promoter constructs (DNA5’-R and DNA5’-S) were transfected with or without CncC/Maf constructs into sf9 cells. Data are the mean ± SD of three independent assays. The data were analyzed using Tukey’s HSD. Letters a, b, c and d denote significant differences at p < 0.05