Fig. 7

Cis-acting mutations in the promoter region enhanced the expression of CYP321B1 gene. A Phylogenetic relationship of CYP321B1 promoter sequences from the susceptible and resistant strains. The phylogenetic tree was inferred using maximum likelihood using MEGA5. Nodes with distance bootstrap values (1000 replicates) are shown. B Analysis of the activity of the CYP321B1 resistant promoter (DNA5’-R) and susceptible promoter (DNA5’-S). Error bars display SD. Letters to the right of bars denote significant differences at p < 0.05 (ANOVA with post-hoc Tukey HSD). C The activity of progressive 5’ deletion constructs of CYP321B1 promoter from the susceptible and resistant strains. Error bars display SD. Letters to the right of bars denote significant differences at p < 0.05 (ANOVA with post-hoc Tukey HSD). D The luciferase activity of progressive 5’-deletion constructs from −353 to −167 of CYP321B1 promoter. The average relative luciferase activity and standard errors of three independent experiments are presented. Different letters on the right of error bars indicate significant differences based on ANOVA with Tukey’s HSD multiple comparison test (p < 0.05)