Fig. 6

Mutations in CncC/Maf binding sites block the ability of these transcription factors to regulate the expression of CYP321B1. A Schematic representation of mutated CYP321B1 promoter constructs. B Analysis of CYP321B1 promoter with the mutation of CncC/Maf binding sequence. The CYP321B1 promoter constructs with or without mutations were transfected with CncC/Maf constructs into sf9 cells. The cells were harvested at 48 h after transfection, and the luciferase activities were measured. The data were shown as the mean ± SD of three independent transfections. The results were analyzed using Student’s t-test and significant differences (p < 0.05) are indicated with an asterisk