Fig. 3

Reduction of TE-derived 21nt RNAs in the Dcr2 mutant does not correlate with differential TE expression. A miRNA-adjusted reads per million mapped reads (RPM) mapping to the different TE orders in the midgut (left plot) and ovaries (right plot) of Dcr2 mutant and control mosquitoes. Error bars denote one standard deviation. P-values shown above the bars were generated with Welch’s t-test. B Ratios between miRNA-adjusted 21nt RNAs expressed in transcripts per million (TPM) and RNA-seq TPM in the control mosquitoes for individual TE families depleted (Down), non-differentially expressed (Not DE), and enriched (Up) in the Dcr2 mutant within each TE order for midguts (top row) and ovaries (bottom row). P-values shown above the graphs were generated using Wilcoxon rank sum test (*p < 0.05, **p < 0.01, ***p < 0.001, NS = non-significant). Families with no detected transcripts were excluded. Families with detected transcripts but no 21nt RNAs detected are shown below the dashed line