Fig. 7

eloF mutations cause partial feminization of pheromone profiles in male D. prolongata. A Schematic diagram of two CRISPR mutant strains: one strain with a 45 bp deletion (“eloF[-] Δ45”) and the other with an early stop codon (“eloF[-] early stop”). Partial eloF locus is shown in green, first and part of second exon are in yellow, and the positions of the two guide RNAs used to generate these mutations are in cyan. The orientation of all features is indicated by arrows. Nucleotide sequences and their translations are shown, with deleted (dashed lines) and surrounding sequences zoomed in to show the amino acid changes. B GC traces of representative (closest to ellipse center) samples for each sex * genotype combination, with male signals (in blue) inverted relative to female signals (in red). Three 9-Monoenes (9T, 9P, 9H) that are most sexually dimorphic in wild-type D. prolongata are labeled, with two corresponding external standards (nC26, nC30) labeled in gray. C PCA ordination of logarithm transformed CHC abundances, partitioned by genotype. Axes are the first two principal components extracted from the variance–covariance matrix of 18 consensus CHCs (Additional file 6: Table S2), with the % variance explained in parenthesis. The first two principal components collectively explain 90% of variation. Points, color-coded by genotype, represent samples, with females in open symbols and males in filled symbols. Ellipses represent 95% confidence regions constructed by bivariate t-distribution. Gray points representing the samples of other genotypes are embedded in each panel as a reference, with wild-type males and females indicated by open ellipses