Fig. 5
Structure and expression of the 5-elongase cluster. A All 5 elongases show a concerted expression increase in D. prolongata males. Dot plots showing normalized expression levels of each gene (RNA-seq data in log2 cpm). For each group, four biological replicates are represented by jitter points, color-coded by species. Males are in filled symbols; females are in open symbols. *** P < 0.001, ** P < 0.01, * P < 0.05. The structure of the ~ 14 kb genomic neighborhood is displayed on top. Numbers above the consensus sequence constructed from the reference genomes of D. prolongata and D. carrolli are coordinates showing the alignment length. Feature annotations are shown with green boxes representing genes, yellow boxes representing CDS, and the orange box representing a predicted ORF in the honghaier insertion, a TE-like repetitive sequence colored in red. The direction of all features is indicated. B The genomic organization of the 5-elongase cluster is conserved. Multiple alignment of DNA sequence across seven species, with species phylogeny on the left and consensus sequence at the bottom. Numbers above all sequences are coordinates showing the length of the consensus (12,702 bp) or alignment (20,139 bp). For each species, site-wise disagreement with the consensus is represented in a vertical gray line for nucleotide substitutions, a vertical black line for nucleotide insertions, and a horizontal line for nucleotide deletions. Feature annotations are displayed as in (A), with the additional purple box representing an antisense RNA. Percent identity per nucleotide across all species is displayed below the consensus sequence, with green indicating perfect (100%) agreement, yellow indicating intermediate (30–99%) agreement, and red indicating low (< 30%) agreement