Fig. 2
Differential expression analysis reveals strongly male-biased expression of eloF in D. prolongata. Volcano plots showing genes with differential expression between D. prolongata males and females (A), differential expression between D. prolongata and D. carrolli males, and interaction effects between species and sex (C). The interaction effects in (C) indicate that either the magnitude of sex differences varies between species, or the direction of sex bias is flipped between species. The x-axis is the log2 fold difference, and the y-axis is the negative log10 of FDR-adjusted P values. Numbers of genes that pass the FDR < 0.05 cutoff for biased expression in either direction are indicated in boxes. D Venn diagram showing candidate gene selection criteria, with 53 final candidates. Numbers of differentially expressed genes (FDR < 0.05) are labeled in parentheses for each one-way comparison