Fig. 1

Experimental strategy of the phosphorylation modification panel in three representative periods during SE. A Workflow of phosphorylated proteome experiments in three representative periods of cotton SE (non-embryogenic calli, NEC, cultured for 5-6 weeks; primary embryogenic calli, PEC, cultured for additional 2-4 weeks; globular embryos, GE, cultured for additional 4-6 weeks). Scale bar = 1mm. B Principal component analysis (PCA) of the three staged samples. C Mass delta of all identified phosphopeptides. Three biological replicates are provided for each sample stage (NEC, PEC, and GE)