Table 1 Sequences of primers used in this study
Primer | Nucleotide sequence | Note |
|---|---|---|
EF1a | F: 5’- ACTGGAACCTCCCAAGCCGAT-3’ | Reference gene |
R: 5’- TTTACACCAAGCGTGTAGGCGAG-3’ | ||
PLRP2-like | F: 5’- GTGCTTATGAGGTCTGGGTTC-3’ | RT-qPCR |
R: 5’- GACAGACTGCTGGTACATCTTAC-3’ | ||
siRNA | F: 5’- CUGUUUACAGAGUCAUUACTT-3’ | Interference |
R: 5’- GUAAUGACUCUGUAAACAGTT-3’ | ||
Probe | F: 5’- CCATCTGACGCTTTGTTTG-3’ | In situ hybridization |
R7: 5’- TACGACTCACTATAGGGGAGTG ACCGGAAGTAGTTAAG-3’ | ||
PLRP2-like | F: 5’- AGTCCGGACTCAGATCTCGAGGC AAGTAGACGGATCCCGG-3’ R7: 5’- GGATCCCGGGCCCGCGGTACCC TGACAGTAGGGAGAGTGACCG-3’ | Overexpression |