Fig. 3

Energy changes in scallop heart after heat exposure. A ATP content variation in nine tissues (including heart, hemolymph, mantle, kidney, gill, hepatopancreas, gonad, muscle, and foot) of scallop in control (NG) and heat exposed groups (MG and DG). The pink and purple bars represent the percentage of energy consumption from NG to MG, and MG to DG out of the total energy consumption from NG. The blue bars represent the residual energy of ATP content. B The relative percentage of Mito-Tracker Green fluorescence levels (left), and the ratio of the red fluorescence to the green fluorescence that marked by JC-1 (right). C Representative mitochondrial status revealed by TEM analysis. The white rectangle framed and zoomed the representative mitochondria in NG, MG and DG. D The bubble shape represents the relative expression of mitophagy marker gene in 16 cell clusters from NG to MG and MG to DG. The significance and regulatory trend are illustrated by the size and color legend, respectively