Fig. 2

scRNA-seq analysis of heat untreated/treated scallop hearts. A Scallop heart collection from control and heat exposure groups, and sequenced with the microfluidics via 10 × Genomic platform. B UMAP plot analysis of 16 cell clusters in control (NG) and heat exposure (MG and DG) groups. C The heatmap (left) representing the top10 enriched genes among the 16 clusters. The cluster numbers correspond to the cluster shown in UMAP visualization. The umap (right) shows the expression patterns of the selected marker genes for each cell type and each dot in the graph represents a cell. The red rectangle emphasizes the umap of cardiomyocytes. D Differentially expressed genes (DEGs) and (E) relative abundance in 16 cell cluster of heart cells after heat exposure