Fig. 2

5hmC profiles in liver metastasis and colon are similar. Colour scheme: yellow normal colon tissue from CRC patients (NC); light blue primary tumour in colon (TC); dark blue metastasised to liver tumours (LT); red normal liver (NL). A Principal component analysis plot showing distinct clustering between NC (n = 9), TC (n = 8), NL (n = 5) and LT (n = 5). B Upset plot showing the overlap of 5hmC peaks in normal and tumour tissues. C Heatmap of 5hmC profiles during CRC tumour progression; first four columns depict average 5hmC levels in each of NL, LM, CT and NC. Subsequent columns depict individual patient samples grouped by tissue type. Four distinct clusters are present: group 1 5hmC presence in all samples; group 2 5hmC present in NL only; group 3 liver metastasis with similarities to both NC and NL; and group 4 recovery of 5hmC in LT matching NC. Star * highlights patient sample LT51, which looks very similar to normal liver