Fig. 2

Split CRISPR/Cas9 system activity. A A simplified diagram showing the positions of gRNA targets within the transformer gene. The universal exons are shown in light grey, whilst the male-specific exons containing premature stop codons are shown in dark grey. B, C Stack graphs showing sex phenotype distributions of trans-heterozygous F1 s resulting from crosses between Cas9-expressing strains (DmPub.2; DmPub.4; CcPub.1) and dgRNA-expressing strains (GuideA.1–2; GuideB.1–2) whereby the Cas9 was supplied maternally (B) (n = 2581) or paternally (C) (n = 1966). No females were found among the 4,577 DsRed +/GFP + flies screened across the 24 cross types. D Representative images of newly-eclosed F1 XX intersexes alongside wild type male and female controls. Male features (genitalia and orbital bristles) are indicated with blue arrow heads; female features (ovipositor and absence of orbital bristles) are shown with pink arrow heads; and intermediate intersex-unique genitalia are highlighted with purple arrow heads