Fig. 6

Differential gene expression analysis and functional enrichment analysis of CM2 and CM4 in the ADO2 and WT groups. a Heatmap displaying the expression levels of the top 20 upregulated genes and top 20 downregulated genes in the CM2 cell cluster between the ADO2 and WT conditions, ranked by the log2 fold change. b GO analysis of 111 downregulated genes in CM2. The horizontal coordinate represents the GeneRatio, the vertical coordinates shows the GO term, the color of the circle corresponds to the magnitude of the p.adjust values, and the size of the circle indicates the number of differentially expressed genes. c Heatmap displaying the expression levels of the top 20 upregulated genes and top 20 downregulated genes in the CM4 cell cluster between the ADO2 and WT conditions, ranked by the average log-fold change. d GO analysis of 145 downregulated genes in CM4. The horizontal coordinate represents the GeneRatio, the vertical coordinate shows the GO term, the color of the circle corresponds to the magnitude of the p.adjust values, and the size of the circle indicates the number of differentially expressed genes. e The expression patterns of downregulated genes, which were enriched in the pathways of “positive regulation of macrophage activation,” “positive regulation of NIK/NF-κB signaling,” “positive regulation of NF-κB transcription factor activity,” and “Toll-like receptor binding” in CM2 and CM4 subsets, across all six subtypes of classical monocytes