Fig. 5

Characterization of monocyte subpopulations and gene expression in the ADO2 and WT groups. a UMAP projection of monocytes within the 14 identified distinct cell clusters, with monocytes labeled in blue. b Pie charts illustrating the proportions of classical monocytes and nonclassical monocytes in the WT and ADO2 groups. c UMAP projection of classical monocytes within the identified monocyte clusters, with classical monocytes labeled in blue. d The visual representation generated through flow cytometry, illustrating the proportion of monocytes within the bone marrow of the ADO2 group. e The visual representation generated through flow cytometry, illustrating the proportion of monocytes within the bone marrow of the WT group. f The visual representation generated through flow cytometry, illustrating the proportion of classical monocytes within the monocytes in bone marrow of the ADO2 group. g The visual representation generated through flow cytometry, illustrating the proportion of classical monocytes within the monocytes in bone marrow of the WT group. h Violin plots depicting the expression levels of the top three differentially expressed genes for each of the six subtypes of classical monocytes identified through unsupervised clustering