Fig. 1

Generation of the ADO2 mouse model and characterization of disease features. a Process of the ADO2 mouse model construction and Sanger sequencing chromatogram confirming the p.R284W (CGG to TGG) mutation. b X-ray examination of ADO2 heterozygous mice and WT mice revealing increased cortical bone thickness and narrowed bone marrow space in ADO2 heterozygous mice. c Histological examination of femur bone sections stained with hematoxylin and eosin (HE) showing increased cortical bone thickness (indicated by asterisks) and thicker trabeculae (indicated by triangles) in ADO2 heterozygous mice compared to WT mice. d Region of interest (ROI) outlined on the maximum intensity projection (MIP) image from PET imaging, depicting the left tibia in ADO2 and WT mice for sodium fluoride metabolism imaging with PET/CT. e In ADO2 mice (N = 3), the maximum standardized uptake value (SUVmax) was significantly lower than that in WT mice (N = 3), with statistical significance determined using a t test (* P < 0.05)