Fig. 1
From: Rare but specific: 5-bp composite motifs define SMAD binding in BMP signaling
SMAD-MH1 domains bind different SMAD binding elements with similar affinities: a EMSA experiments are performed testing the binding of human SMAD1/3/4-MH1 domains to palindromic GC-SBE (pGC-SBE), non-palindromic GC-SBE (npGC-SBE), and SBE. Protein concentrations (µM) are shown on top of the EMSA. Abbreviations for the DNA oligonucleotides and dsDNA sequence are shown above. Single and double SMAD-MH1 binding to dsDNA is indicated with black triangles. Double binding at saturating concentrations (above 1.3 µM) can be the result of unspecific binding (representative blot). b Fractions of bound SBE probes plotted against SMAD-MH1 concentrations, with Hill equation curve fitting of data obtained. Kd values are indicated; n = 2 independent experiments