Fig. 6

CBL is a direct target of miR-320a. A The candidate E3 ligase targeted by miR-320a. B siRNA was transfected into A549 cells. The expressions of AMPK was analysed by Western blotting. C Predicted binding site of miR-320a and CBL 3'-UTR. D Luciferase activities in HEK-293 T cells cotransfected with miR-320a mimic and luciferase reporter constructs containing WT or Mut-type UTR of CBL. E A549 cells were transfected with control inhibitor, miR-320a inhibitor, NC mimics, and miR-320a mimics, the expression of miR-320a was detected by qRT-PCR. F qRT-PCR was used to detect the mRNA levels of CBL after transfection with the control inhibitor, miR-320a inhibitor, NC mimics, and miR-320a mimics. G-H A549 cells were treated with miR-320a mimic and EVs, and then cells were collected for Western blotting to show CBL expression. Blots were representative of six independent experiments. All data are presented as the mean ± SEM of n = 6. ***p < 0.001, **p < 0.01, *p < 0.05, ns, no significance