Fig. 4

ADSCs-derived EVs relieve ALI via delivery of miR-320a. A The expressions of different miRNAs were analyzed by qRT-PCR in ADSCs-derived EVs. B qRT-PCR was used to determine EVs-derived miR-320a expression in the control, RNase A, and RNase A plus Triton X-100 groups. C miR-320a expression in mice lung tissues was detected by qRT-PCR. D Timeline diagram of intranasal delivery and intravenous injection of WT-EVs or miR-320a KO-EVs. E H&E staining of lung tissues. Scale bar, 400 μm. F The lung injury scores. G Immunostaining analysis of TNF-α expression in the lung tissues of mice. Scale bar, 100 μm. H Quantitative analysis of TNF-α-positive cells in the lung tissues. I BALF protein concentration was determined by BCA (n = 6). J and K SA bacterial loads in BALF and lungs from mice treated with PBS, WT-EVs, and miR-320a KO-EVs. L-O The expression of IL-1β, IL-6, MCP-1, and TNF-α in BALF was tested by ELISA. HE, IHC images were representative of six independent experiments. All data are presented as the mean ± SEM of n = 6. ***p < 0.001, **p < 0.01, *p < 0.05, ns, no significance