Fig. 1

ADSCs relieve SA-induced ALI in vitro. A Changes in cellular oxidative phosphorylation were detected by extracellular flux analysis. B and C ATP production and mtDNA expression in A549 cells. D Mito Tracker Red staining of mitochondria in A549 cells. Scale bar, 20 μm. Mito Tracker Red: red, DAPI: blue. E The quantitative analysis of mitochondrial fluorescence intensity. F The mitochondrial potential was observed via JC-1 staining. G The red to green fluorescence ratio was recorded to quantify the mitochondrial potential (rate). Scale bar, 20 μm. H ROS levels were assessed. I The relative fluorescence intensity ratio shown in (H) was analyzed. Scale bar, 20 μm. J qRT-PCR was performed to determine mRNA levels of mitochondrial fragmentation-related genes, including Drp1, Mfn1, and Mfn2. K Cell proliferation was determined by the CCK-8 assay. L Caspase 3 activity was measured in different groups. M The expression levels of Caspase 3, Bcl-2, and Bax were analyzed by Western blotting. Fluorescence images and blots were representative of six independent experiments. All data are presented as the mean ± SEM of n = 6. ***p < 0.001, **p < 0.01, *p < 0.05, ns, no significance