Fig. 3

Interplay between epigenomic and transcriptomic variation. (a, b) Gene expression variation and (c, d) alternative splicing variation partitioning, showing the proportions of gene expression or splicing variation explained by CpG methylation and miRNA expression, based on the adjusted R² of redundancy analyses (RDA) in (a, c) brain and (b, d) liver. Numbers outside the circles refer to total percentage of variation (adjusted R²) explained by both CpG methylation and miRNA expression (top, full model), or by only CpG methylation or miRNA expression (reduced models). Percentages inside circles indicate pure contributions to gene expression or splicing level resulting from partial RDAs that isolate the effect of one single explanatory variable. Percentages within intersections indicate shared contribution across two variables to gene expression or splicing variation. ANOVA-like permutation tests were calculated on total and pure contributions. NT, non-testable; NS, non-significant; ***, p < 0.001; **, p < 0.01; *, p < 0.05. (e, f) Venn diagrams of DEGs, DSGs, DEmiR target genes and DMC-associated genes in (e) brain and (f) liver