Fig. 4

MiT family transcription factors Tfe3, Mitf, and Tfec regulate the expression of the tyr and dct genes. A–B show the nucleotide frequency of different bases at various positions within the DNA binding motifs for Mitf and Tfe3. Data were obtained from JASPAR. C The DNA binding motifs for Mitf and Tfe3 in the promoter region of tyr and dct (2000 bp upstream of the transcription start site, TSS). The blue numbers indicate the distance (in base pairs) from the TSS. Data were obtained from JASPAR and NCBI. D–E Present dual-luciferase assay results for the regulation of tyr and dct expression by Mitf, Tfe3, and Tfec (n = 4). *** indicates a P value < 0.001, as determined by an unpaired t test