Fig. 5
From: Label-free metabolic fingerprinting of motile mammalian spermatozoa with subcellular resolution
Metabolic fingerprints of spermatozoa and midpieces differed between fertile and subfertile males. A Representative FLIM images of sperm cells and midpiece mitochondrial compartments. Object-based phasor fingerprints B and C show two distinct clusters corresponding to PWD (fertile) and B6PF1 (subfertile) males. Sperm phasor fingerprints of PWD (4 males, 107 cells) and B6PF1 (2 males, 91 cells) were generated manually. U-Net segmented midpieces of PWD (4 males, 1016 objects) and B6PF1 (2 males, 957 objects) automatically. See Additional file 6: Fig. S6 for head and principal piece fingerprints. Scale bar, 20 μm