Fig. 4
From: Label-free metabolic fingerprinting of motile mammalian spermatozoa with subcellular resolution
Object-based phasor metabolic fingerprints distinguish sperm midpieces of three mouse strains. A Representative FLIM images of PWD (M. m. musculus), B6 and C3H (M. m. domesticus) depicting whole sperm cells and U-Net-segmented midpiece subcellular compartments, respectively. Metabolic fingerprints in B and C are different for each strain at the single-cell and subcellular midpiece levels. Sperm phasor fingerprints of PWD (2 males, 149 cells), B6 (3 males, 207 cells), and C3H (6 males, 632 cells) were generated manually. U-Net segmented midpieces of PWD (2 males, 1426 objects), B6 (3 males, 1646 objects), and C3H (6 males, 3208 objects) strains. D Phasor g values of sperm and midpiece fingerprints correlated significantly with male’s reproductive success given as litter size. Every filled circle represents the mean value for one male, color-coded by strain as the single sperm cell and midpiece values above