Fig. 2
From: Label-free metabolic fingerprinting of motile mammalian spermatozoa with subcellular resolution
In situ 2P-FLIM NAD(P)H imaging unveils the native metabolic state of motile spermatozoa. A Greyscale image of NAD(P)H fluorescence intensity signals (left) and color-coded image of NAD(P)H fluorescence lifetimes (right). Note the increased cellular autofluorescence in the mitochondria-rich midpiece in both images, as well as in the zoom insets displaying the spermatozoon. B Object-based phasor plot and C the individual points display the metabolic states of motile spermatozoa and subcellular compartments including the midpiece, head, and principal piece. H, head; M, midpiece; P, principal piece. Scale bar, 20 μm