Fig. 5
A. cf. solaris RGP1 is a ligand for A. cf. solaris RXFP/LGR3 but not for A. cf. solaris LGR4. A AsolRGP1 induces a dose-dependent increase in CRE reporter gene activation in CHO-AsolRXFP/LGR3 cells. The potency of AsolRGP1 at AsolRXFP/LGR3 was ~ 16 nM (pEC50 = 7.80 ± 0.18). AsolRGP2 induced a small but significant response but no clear dose–response, while the negative control H2 relaxin induced no response. B, C Neither AsolRGP1 nor AsolRGP2 induced CRE reporter gene activation in HEK-293 T cells transfected with AsolLGR4 (long) or AsolLGR4 (short). The graphs show the pooled data from at least three assays performed in triplicate within each assay (n ≥ 3) expressed as mean values with error bars (S.E.M.). The raw data from all three experiments are shown in Additional file 17: Dataset S10