Fig. 10

BMP signaling is broadly active in medusozoans, the scyphozoan jellyfish Aurelia and the cubozoan jellyfish Tripedalia. A Scheme of the life cycle of Aurelia coerulea. B–D’ pSMAD1/5-positive nuclei in the ephyra (B–B’) central disc (cd), strong staining in the manubrium (ma) is likely unspecific (non-nuclear), C–C’ lappet and rhopalia. D–D’ vasa2-staining in the region of the gastric filaments (yellow arrowhead) shows no overlap with pSMAD1/5-positive nuclei (blue arrowhead). E Scheme of the life cycle of Tripedalia cystophora. F Broad pSMAD1/5 activity in the umbrella and velarium. G Tripedalia gonad is pSMAD1/5-positive. H–H” Germ cells in the Tripedalia gonad are pSMAD1/5- and vasa-positive. All stainings were performed two times independently with 4 animals imaged. Scale bar 50 µm (white) and 25 µm (B’, yellow). ma—manubrium, cd—central disc, vla—velar lappet, mla—marginal lappet, gf—gastric filament, gfb—gastric filament base, um—umbrella, nc—nematocyst wart, vel—velarium, pe—pedalium, gon—gonad