Fig. 3

Microbiome associations with processes of wearing, washing, dry-drying and humid drying. a Relative abundances of the 20 most common genera and b Significant associations (FDR < 0.05) of metagenomics genera detected by Microbiome Multivariable Associations with Linear Models’ (MaAsLin2’s) default linear mixed effects model. Only associations significant in at least two sample type pair comparisons are shown. Detected associations are adjusted for participant as random effect and sample origin as fixed effect. Values are log-transformed relative abundances with half the minimum relative abundance as pseudo count. Complete linkage clustering of Pearson correlation coefficients was used to hierarchically cluster the features. Dots indicate the significant level of the FDR-adjusted p-values: < 0.001 “●”, < 0.05 “○”. Legend: A_T1: axilla after wearing the T-shirt for 48 h, T: worn T-shirt, T_w: worn washed T-shirt, T_dd: dry-dried worn T-shirt, T_hd: humid-dried worn T-shirt