Fig. 3
From: Anemonefish use sialic acid metabolism as Trojan horse to avoid giant sea anemone stinging
Survival rates and sialic acid production changes during A. ocellaris larval development. A Combined graphs showing in red the survival rates (percentage) of A. ocellaris larvae sampled at different developmental stages [37] and in blue Neu5Ac levels (expressed in ng/100 µg of protein) of larvae sampled before metamorphosis (stage 2) and during metamorphosis (stage 4 and 6). A one-way ANOVA (p value displayed on the graph) followed by a Tukey HSD test was performed on Neu5Ac levels to compare each developmental stage. Stage with different letter has significantly different Neu5Ac levels. B Expression levels of genes involved in sialic acid synthesis (gne, nans, nanp, cmas), transport (slc35a1, slc17a5), removal from glycoconjugate (neu1, neu3, neu3.1), and degradation (npl). Genes written in bold are significantly differentially expressed between pre-metamorphosis stage (S1 and/or S2 and/or S3) and metamorphosis stages (S5 and/or S6 and/or S7) (see Roux et al. [37] for the description of gene expression analysis method). Data are presented in Additional file 6: Tables S3 and S4