Fig. 5

CDK1 regulates break-induced replication and RAD51-mediated homology-directed repair in MII oocytes. A The CDK1 inhibitor RO-3306 suppressed the number of EdU foci in SN DSB oocytes but did not affect the total γH2A.X volume. Statistics performed by Kramer-Nemenyi test. B CDK1 inhibitor RO-3306 suppressed the RAD51 foci formation in DSB SN oocytes. Statistics performed by Kramer-Nemenyi test. C, D RO-3306 decreased the proportion of oocytes with strong EdU signals when the DSBs were introduced at IVM 2 h C or IVM 14 h D. Statistics performed by Tukey HSD test. E(D) RO-3306 had no significant effect on the number of GFP-53BP1 foci in DSB MII oocytes. The oocytes were treated with 10 μM of Bleomycin for 1 h and released from Bleomycin for 1 h. Statistics performed by Kramer-Nemenyi test. F Compared with those in oocytes matured in M2 medium, the protein levels of CDK1 and CCNB1 in oocytes matured in DMEM or M2 + glucose + pyruvate + FBS (M2 + G.P.F.) medium decreased. Cofilin was used as an internal reference protein. G Pronucleus-like nuclei can be found in DMEM-matured oocytes and RO-3306-treated MII oocytes. Scale bar, 10 μm. Significance markers: *, p < 0.05; **, p < 0.01; ns, p > 0.05. Repeat number and sample size are indicated by the number of dots in the dot plots