Fig. 4

RAD51-mediated homology-directed repair but not nonhomologous end joining is suppressed in MII oocytes. A GFP-RAD51 foci can be found in Bleomycin treated SN oocytes. B In IVM 2 h oocytes, GFP-RAD51 foci formed in both DMEM and M2 cultured DSB oocytes. C RAD51 foci associated with EdU signals in both DMEM and M2 cultured DSB IVM 2 h oocytes. D The formation of GFP-RAD51 foci was suppressed in MII oocytes which were matured in DMEM. Statistics performed by Fisher’s exact test. E GFP-RAD51 foci formation correlated with break-induced replication (BIR). BIRs are marked by EdU signals at chromosome regions. F The DSB caused GFP-53BP1 foci formation was inhibited in GV and IVM 2 h oocytes but activated in IVM 14 h MII oocytes. G There was no significant difference in the number of GFP-53BP1 foci between M2- and DMEM-cultured oocytes after DSB induction. Scale bar, 5 μm for E and 10 μm for others. Significance markers: *, p < 0.05; **, p < 0.01; ns, p > 0.05. Statistics performed by Kramer-Nemenyi test. Repeat number and sample size are indicated by the number of dots in the dot plots