Fig. 3

Break-induced replication in MII oocytes is regulated by the culture medium. A Break-induced replication in in vivo oocytes (2 h or 14 h after hCG injection), DMEM-cultured cumulus-enclosed oocytes (CEOs, IVM 14 h), DMEM-cultured denuded oocytes (DOs, IVM 14 h), and M2-cultured DOs (IVM 14 h). EdU strong signals could be detected in in vivo hCG 2 h DSB oocytes and in M2 cultured IVM 2 h DSB DO oocytes. Statistics performed by Fisher’s exact test. B IVM medium had no obvious effect on the polar body extrusion (PBE) rate of oocytes. Statistics performed by Tukey HSD test. C Break-induced replication is suppressed in M2 + G.P.F. and M2plus group oocytes. Statistics performed by Tukey HSD test. Gl, glucose; Py, sodium pyruvate; G.P., glucose + sodium pyruvate; G.P.F., glucose + sodium pyruvate + FBS; M2plus, M2 + glucose + sodium pyruvate + FBS + essential/nonessential amino acids. Scale bar, 10 μm. Significance markers: *, p < 0.05; **, p < 0.01; ns, p > 0.05. Repeat number and sample size are indicated by the number of dots in the dot plots