Fig. 7
The critical sex-related candidate gene foxl2 in silver arowana. A Relative expression of sex-related genes at three developmental stages. See more details about classification of each stage in Table S9 of Additional file 9. B qPCR validation of target genes including foxl2. Asterisk (*) represents a significant difference (p < 0.05). C Proposal of the possible signaling pathways for foxl2 in silver arowana gonads. D A phylogenetic tree of foxl2, indicating its relative conservation among various vertebrates. E Alignments of foxl2 and flanking sequence among representative species within the order Osteoglossiformes