Fig. 4

Dendritic growth cones of Mov10 Deletion hippocampal neurons have less polymerizing plus-end microtubules but higher acetylation. A Representative images of the EB3-comets in the dendritic growth cones of DIV2-4 WT and Mov10 Deletion (DEL) hippocampal neurons over time (seconds). A white arrow identifies a single EB3-comet whose trajectory over time is indicated by a red line. B Rate of EB3 comet formation is the number of comets/second (Y axis). C Traveling rate of EB3 comets is the distance in μm/second (Y axis). Number of neurons imaged from each genotype is indicated in the bars. P-values were calculated using Mann–Whitney U test. D Representative images of DIV7 WT and Mov10 Deletion hippocampal neurons stained for acetylated tubulin (AcTUB) and for alpha tubulin (AlTUB). Scale bar = 20 μm. E Fluorescent intensity of AcTUB and AlTUB and ratio of AcTUB/AlTUB in WT and Mov10 Deletion DIV7 hippocampal neurons. Data are shown as mean ± SEM. P-values were calculated using two-sided unpaired Student’s T-test. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. “n” = number of neurons in the genotypes indicated from N = 3 litters cultured separately