Fig. 3

Increased distal dendritic arborization in Mov10 Deletion hippocampal neurons. A, B Representative images of confocal z-stacks of MAP2 (red) and PROX1 (green)-stained WT and Mov10 Deletion (DEL) DIV14 neurons analyzed using Sholl analysis: the number of intersections (Y axis) and the distance from the soma (mm) (X axis). A PROX1 + granule cells B PROX1.⁻ pyramidal neurons. Scale bars represent 20 μm. “n” = number of neurons cultured from N = 3 litters cultured separately. C Percentage of PROX1 + granule cells and PROX1- pyramidal neurons in the DIV14 Mov10 Deletion and WT primary hippocampal cultures. D Length (μm) of the longest dendrites of the DIV14 WT and Mov10 Deletion hippocampal neurons. E Number of nodes in the dendritic arbors of the DIV14 WT and Mov10 Deletion hippocampal neurons. F Soma area of the DIV14 WT and Mov10 Deletion hippocampal neurons. G Schematic representation of the dendritic phenotype observed in Mov10 Deletion hippocampal pyramidal neurons. P-values were calculated using Mann–Whitney U test. Data are shown as mean ± SEM. *p-value < 0.05