Fig. 2

Wolbachia increases the longevity of flies of the w^k background line infected with certain filamentous fungal entomopathogens. Flies of each given background and sex were systemically infected with the indicated pathogen. Infections were performed with either a Beauveria bassiana, b Metarhizium anisopliae, c Clonostachys rosea, or d Trichoderma atroviride. Infections of all groups were performed side-by-side, along with those of the w¹¹¹⁸ background line (Figure S2), with at least two blocks of infections performed on different days. Each line represents a total of 60 flies. Sham controls were performed with sterile 20% glycerol. Full statistics, available in Table S1, were done with a Cox mixed effects model. Controls for panel 2a are the same for Figure 1, and the panels in 2b–d are the same because they were performed concurrently in the same background.