Fig. 1

Mapping activating and repressing histone modifications in human GIC as compared to syngeneic iNSC. a Average heat map of ChIP Seq dataset around transcription start sites (TSS) and annotated genes for the 10 patients. Colours show read density. b Pie charts show proportion of peaks linked to each histone modification (HM) in GIC (left) and iNSC (right) in all patients. Heatmaps represent Fisher’s exact test statistical analysis and fold change of GIC ChIP-peaks upon iNSC ChIP-peaks for each HM. c Volcano plots represent the comparative analysis of differentially bound sites between GIC and iNSC for the four HM in all patients. Only significantly differentially bound sites are shown (FDR < 0.05). Results are represented as differential log fold change of GIC upon iNSC [Log2(GIC)-Log2(iNSC). Bound sites only found in GIC are shown as purple dots (log(FC) > 1) and bound sites found only in iNSC are shown as pink dots (log(FC)M < − 1). Bound sites with a − 1 < log(FC) < 1 are shown as black dots. d Distribution of ChIP Seq peaks’ genomic annotations for each HM GIC and iNSC. Heatmap represent Fisher’s exact test statistical analysis and fold change of ChIP Seq peaks between GIC and iNSC. e Percentages of genes common, only found in GIC (GIC specific) and only found in iNSC (iNSC specific) in each HM. f Schematic representation of HM redistribution in GIC as compared to iNSC and their targeted pathways. Red and blue shades in the donut represent activated and repressed signalling pathways, respectively