Fig. 4

Evolution of serpins in the tick I. ricinus. A Serpin expression profile. The expression heatmap is based on log10(TPM) (transcripts per million) calculated for the respective transcriptomes: SYN, synganglion; SG, salivary glands; OV, ovary; MT, Malpighian tubules; MG, midgut; FB.T, fat body/trachea; UF, unfed females; F, fully fed females; WB, whole body. B Consensus phylogenetic tree of serpins from I. ricinus. Sequences were aligned as proteins; signal peptides and variable reactive center loops were removed before the analysis as well as non-informative positions. Edited protein sequences were analyzed by maximum likelihood method and JTT matrix-based model, and bootstrap method with 1000 replications was used to calculate the reliability of tree branches. Only branches with bootstrap value equal or higher than 50% are shown. Mono-exonic serpins are shown with an orange dot. Specific clades are represented by colored areas in the phylogenetic tree, using the same background color for sequence labels in the heatmap